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Vimentin 抗体

Name: Vimentin 抗体
SKU: ABIN112184
Availability: OutOfStock

This anti-Vimentin antibody is a 小鼠单克隆 antibody detecting Vimentin in WB, IF, FACS, IHC (p) 和 IHC (fro). Suitable for 人, 大鼠, 小鸡, 绵羊和 Potoroo.

产品编号 ABIN112184

发货至: 中国

Quick Overview for Vimentin 抗体 (ABIN112184)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

适用

人, 大鼠, 小鸡, 绵羊, Potoroo

宿主

小鼠

克隆类型

单克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

Discover our top product VIM Primary Antibody

特异性

The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.

纯化方法

Purified

免疫原

V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.

亚型

IgG1
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

应用备注

This antibody is suitable for Immunoblotting, Immunocytochemistry,Immunohistochemistry on frozen sections and paraffin-embedded tissues and Flowcytometry. Recommended dilutions: Flow cytometry: 1/25-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Immunoblotting applications: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL

缓冲液

PBS with 0.09 % Sodium Azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. Do not freeze working dilutions.

储存条件

4 °C/-20 °C

储存方法

Store the original vial at 2-8 °C. After reconstitution, aliquot and store stock solution or aliquots thereof at -20 °C.
抗原

Vimentin (VIM)

别名

Vimentin

背景

Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.Synonyms: VIM

基因ID

7431

NCBI登录号

NP_003371

UniProt

P08670

途径

Caspase Cascade in Apoptosis